Achyranthis Bidentatae Radix(ABR) is important prescriptions that have been used in oriental medicine for stomatitis and wound healing. The study was done to evaluate the inhibitory effects of cytotoxicity, formation of superoxide on the macrophage and neutrophil, prostaglandins(PGE₂), interleulrins(IL-1β), collagenase activity and synthesis of collagen and DNA.
The results were obtained as follows:
1. ABR was not showed the proliferation difference of human fibroblast and monocyte in 0.01% and 0.001% concentrations to be experimented and in result, it was concluded that they have no cytotoxicity but showed cytotoxicity in 0.1% concentrations.
2. ABR inhibited the formation of superoxide to 48% at the concentration of 0.001% in the mouse monocyte.
3. ABR inhibited the formation of superoxide to 40% at 0.001%, 58% at 0.0001% as compared with control in the human monocyte.
4. ABR inhibited the formation of superoxideto 58% at 0.0001%, 40% at 0.001% in the human neutrophil.
5. ABR was not showed the proliferation difference of human monocyte in all concentrations to be experimented and in result, it was concluded that they inhibited the formation of prostaglandins(PGE₂) in the human monocyte stimulated with E. coli.
6. ABR showed the all concentration of inhibiting the production of interleukins(IL-1β) in the human monocyte stimulated with E. coli.
7. ABR didn´t influence on collagen synthesis and total protein in fibroblasts.
8. ABR inhibited the collagenase activity to 84% at 0.1%, 69% at 0.2%, 76% at 0.5%, 91% at 0.001% respectively.
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